This study provides a framework for the production of Hypermedia Instructional package. It also assessed the effectiveness of hypermedia instructional mode of delivery on students’ performance in Chemistry. This is with a view of improving the learning of Chemistry which may eventually help to improve students’ performance. The developmental study employed a pre-test, post-test control group design. The research sample consisted of 60 private secondary school students in Osun State with an enrolment of 30 students from each of the two schools selected. The students were thereafter assigned to experimental and control groups. The stimulus material used for the experimental group was the Hypermedia Instructional Package, while the Control group received instruction with similar content through the conventional method. The test instrument used was Hypermedia Learning Achievement Test (HLAT). Content and face validity of the instrument used was carried out by experts in the area of tests and measurement. The Cronbach’s alpha reliability coefficient for the HLAT gave a value of 0.72. Data collected were analyzed using descriptive and inferential statistics. Results obtained showed that students exposed to the Hypermedia Instructional Package (HIP) performed significantly better than those exposed to the conventional teaching method (t = 5.458, df = 58, p < 0.05). Results also revealed a significant difference in the retention ability of students in Chemistry between those exposed to the package and those that were not (t= 6.842, df=58, p<0.05). The study concluded that the use of HIP was an intervention which improved students’ performance retention and attitude towards Chemistry.