Emotional empathy, mindfulness, and subjective well-being are essential to understanding human behavior and mental health among students. However, more research is needed to investigate how these constructs interplay within academic contexts. This study explored the hierarchical relationships between emotional empathy, mindfulness, and subjective well-being. The Multidimensional Emotional Empathy Scale (MDEES), The Kentucky Inventory of Mindfulness Skills (KIMS), and the Subjective Well-Being Scale (WeBs) were administered with a sample of postgraduate professional diplomas in teaching students attending Al Ain University in Abu Dhabi campus and Al Ain campus (n = 1545). The results showed that emotional empathy (positive sharing, suffering, feeling for others, and emotional contagion) positively affects physical and eudaimonic well-being. A negative correlation was found between financial and social well-being and other components of emotional empathy, such as emotional attention and responsive crying. Mindfulness significantly improves emotional empathy in components like describing, accepting without judgment, and observing. This study revealed that some components of mindfulness, such as observing and acting with awareness, decrease emotional empathy, such as suffering and feeling for others. Acting with the awareness component in mindfulness decreases positive sharing, responsive crying, and emotional contagion. Future research could explore these relationships further and examine potential cultural differences.